Fig. 5

Caspase-1 inhibitor suppressed Tfh cells and lymphocyte proliferation but did not influence Treg cells in EAMG rats. MNCs were isolated from the lymph nodes of rats in three groups. CD4⁺CD25⁺Foxp3⁺ T cells (a) and CD4⁺CXCR5⁺ICOS⁺ T cells (b) were assessed by FACS as indicated. Proliferation response of lymphocytes derived from three groups were studied on day 43 p.i.. Lymphocyte proliferation was measured by using CCK-8 and OD values at 450 nm subtracted from 630 nm (c). The results are expressed as means ± SD (n = 6 in each group) (*p < 0.05 and **p < 0.01)