Fig. 8
a LC3 immunohistochemistry in neurons after CLP injury and PDTC treatment. Three-color staining for LC3 (green), NeuN (red), and DAPI (blue) demonstrated an increase in LC3 punctate labeling in hippocampal neurons after CLP and PDTC treatment. The immunoreactivity of LC3 was elevated robustly in PDTC + CLP group. b Quantitative immunofluorescence data showed that the number of recognized cytoplasm intense punctate LC3 cells is significantly increased in hippocampal neurons after PDTC treatment. Ten fields for each animal were randomly selected. Data were expressed as mean ± SEM from five independent experiments in each group. n = 5/group. *P < 0.05 vs sham-operated group. # P < 0.05 vs 12 h or Veh + CLP group. Brain sections were viewed under a fluorescence microscope. Insets 400 μm. LC-3 microtubule-associated protein light chain-3, CLP cecal ligation and puncture, PDTC pyrrolidine dithiocarbamate, DAPI 4,6-diamidino-2-phenylindole