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Table 1 List of primary antibodies

From: Age-related increases in amyloid beta and membrane attack complex: evidence of inflammasome activation in the rodent eye

Antigen Antibody Dilution Source Applications
Membrane attack complex (MAC) Mouse monoclonal (clone aE11) 1:50 (IHC) 1:1000 (WB) Dako, Burlington, ON, Canada Immunocytochemistry Western blot
Membrane attack complex (MAC) Rabbit polyclonal 1:500 BIoss, Woburn, MA Immunohistochemistry
Interleukin-18 (IL-18) Rabbit polyclonal 1:100 Santa Cruz Biotechnology, Dallas, TX Immunohistochemistry
Amyloid-beta amino acid 17–24 (Aβ17–24) Mouse monoclonal anti-Aβ17–24 (clone 4G8) 1:400 (IHC) 1:1000 (WB) BioLegend, Dedham, MA Immunohistochemistry Western blot
Phosphorylated NF-κB p65 (Ser 276) Rabbit polyclonal 1:75 (IHC) 1:500 (WB) Santa Cruz Biotechnology, Dallas, TX Immunohistochemistry Western blot
Phosphorylated NF-κB p50 (Ser 337) Rabbit polyclonal 1:500 (WB) Santa Cruz Biotechnology, Dallas, TX Western blot
Caspase-1 Mouse monoclonal 1:1000 R&D Systems, Minneapolis, MN Western blot
Amyloid-beta amino acid 1–16 (Aβ1–16) Mouse monoclonal anti-Aβ1–16 (clone 6E10) 1:2000 BioLegend, Dedham, MA Western blot
GAPDH Mouse monoclonal 1:10,000 EMD Millipore, Billerica, MA Western blot