Fig. 3

rhPDCD5 treatment reduces Th1/Th17 response in EAE mice in vivo. a, c DLN cells were collected from EAE mice treated with OVA or rhPDCD5 (10 mg/kg i.p.) prophylactically and therapeutically at day 25 after immunization and stimulated with PMA plus ionomycin in the presence of brefeldin A for 4 h. CD4⁺IFN-γ⁺ T cells and CD4⁺IL-17A⁺ cells were measured by flow cytometry. b, d Percentages of CD4⁺IFN-γ⁺ T cells and CD4⁺IL-17A⁺ T cells are shown as means ± SEM (n = 10). *P < 0.05, **P < 0.005