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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Recombinant human PDCD5 (rhPDCD5) protein is protective in a mouse model of multiple sclerosis

Fig. 7

rhPDCD5 induces apoptosis of MOG-specific T cells. a Apoptosis of CD4+ T cells was determined and quantified by the percentage of AV positive cells showing an increase in a dose-dependent manner, and representative histograms are shown. b Lymphocytes treated with rhPDCD5 were examined the expression of PDCD5 mRNA and protein levels by RT-PCR and Western blot analysis. Gels have been run under the same experimental conditions. c DLN lymphocytes and splenocytes isolated from donor mice 12 days post-immunization were re-stimulated with MOG and rhPDCD5, and 3 × 106/ml CD4+ T cells were transferred into irradiated naïve mice to induce passive EAE. Mice were then monitored daily for mean clinical score. Two independent adoptive transfer experiments were performed. Data are means ± SEM from mice treated with MOG (n = 8) or MOG+ rhPDCD5 (n = 8). Statistical analysis was performed using repeated measures two-way ANOVA followed by Bonferroni post hoc tests to compare replicate by time. *P < 0.05

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