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Fig. 10 | Journal of Neuroinflammation

Fig. 10

From: Chronic morphine and HIV-1 Tat promote differential central nervous system trafficking of CD3+ and Ly6C+ immune cells in a murine Streptococcus pneumoniae infection model

Fig. 10

Co-localization of chemokine ligands and microglia. Confocal microscope and z-stacked images of the prefrontal cortex brain tissue region showing co-localization of chemokine ligands (Alexa Fluor 488-conjugated anti-mouse IgG; green): a CCL5 and b CXCL12 (Rhodamine Red goat anti-rabbit IgG; red) with microglia (IBA+) rather than astrocytes (GFAP+). Counterstained nuclei of the cells with DAPI (blue). Magnification ×600. Merged images present co-localization of glia (microglia or astrocytes) with chemokine ligand (CCL5 or CXCL12). Scale bar = 10 μm. Magnification of the boxed area ×2400

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