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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Chronic morphine and HIV-1 Tat promote differential central nervous system trafficking of CD3+ and Ly6C+ immune cells in a murine Streptococcus pneumoniae infection model

Fig. 3

Significant trafficking of CD3+ and Ly6C+ immune cell population into the CNS of mice treated with morphine and HIV-1 Tat and co-infected with S. pneumoniae (S.p) at the 5th day of post-pellet implantation. Single-cell suspension was isolated from the perfused brain of treated mice. Flow cytometry analysis was performed to measure the frequency of TOPRO3-CD45highCD11b-CD3+ (T cells) and TOPRO3-CD45highCD11b+Ly6C+ (inflammatory monocytes) in the CNS. a Representative dot plots demonstrating high influx of T lymphocytes and inflammatory monocytes into the CNS of treated mice. The histogram represents quantification of specific immune cell (b CD3+; c Ly6C+) frequency as fold change from the group of untreated mice [basal level (dashed line)] in the CNS. Error bars indicate the standard error of the mean (±SEM) of six animals in each group (n = 6 mice). PP placebo pellet, MP morphine pellet. **P < 0.01

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