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Fig. 9 | Journal of Neuroinflammation

Fig. 9

From: Chronic morphine and HIV-1 Tat promote differential central nervous system trafficking of CD3+ and Ly6C+ immune cells in a murine Streptococcus pneumoniae infection model

Fig. 9

Chemokine ligand (CCL5 and CXCL12) production is significantly high in the prefrontal cortex region of mice chronically treated with morphine, HIV-1 Tat, and S. pneumoniae. Histograms of differential mRNA expression of a CCL5 and b CXCL12 genes using 18S mRNA as internal control in prefrontal cortex brain tissue isolated from the treated mice from all the groups. Histograms of band intensity of chemokine protein synthesis of c CCL5 and d CXCL12 using ImageJ software. e Correlation analysis (R 2) of CNS ligand (CCL5 and CXCL12) expression on the y-axis with peripheral immune cell trafficking (i and iii) CD3+ and (ii and iv) Ly6C+ into the CNS (x-axis) of the wild-type animals treated with chronic morphine, HIV-1 Tat, and S. pneumoniae. f Histograms of mean quantification of chemokine ligand CCL5 and CXCL12 generation in the prefrontal cortex region of wild-type and TLR knockout mice. Error bars indicate the standard error of the mean (SEM) of six different animals from each group (n = 6). PP placebo pellet, MP morphine pellet. *P < 0.05; **P < 0.01

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