Fig. 3

DRα1-mMOG-35-55 treatment increases splenocyte number but does not affect their activation state. a Representative image of spleens of vehicle-treated (n = 8) or DRα1-mMOG-35-55-treated (n = 8) C57BL/6 male WT mice with EAE. b Absolute cell numbers of CD4⁺, CD19⁺, CD11b⁺, and CD11c⁺ cells in spleen. c Frequency of CD4⁺CD69⁺ and CD4⁺CD44⁺ (activated CD4⁺ T cells) in spleen. d Splenocytes from DRα1-mMOG-35-55-treated (n = 4) and vehicle-treated (n = 4) mice were stimulated with PMA (50 ng/ml) and ionomycin (500 ng/ml) for 4 h. CD4⁺ T cells were evaluated for the intracellular expression of IFN-γ or IL-17 by flow cytometry. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, Student’s t test