Fig. 6
From: Cu(II) enhances the effect of Alzheimer’s amyloid-β peptide on microglial activation
Cu(II)-Aβ complex-induced microglial activation depends on NF-κB. a, b BAY11-7082 and SC-514 inhibited Cu(II)-Aβ1–40-induced release of TNF-α or nitric oxide. BV-2 cells were pretreated with BAY11-7082 (10 μΜ) or SC-514 (50 μΜ) for 1 h prior to 24-h stimulation with Cu(II)-Aβ1–40 (5 μΜ peptide). Data are expressed as the means ± SEM of at least three independent experiments. Significance was tested by one-way ANOVA followed by Bonferroni post-test comparisons. *P < 0.05, **P < 0.01 vs control; # P < 0.05 vs Cu-Aβ. c The phosphorylation and degradation of IкB-α and phosphorylation of p65 in BV-2 cells stimulated with Cu(II)-Aβ1–40. The cells were treated with Cu(II)-Aβ for indicated time intervals