Fig. 7
From: Cu(II) enhances the effect of Alzheimer’s amyloid-β peptide on microglial activation
Cu(II)-Aβ complex induces NADPH oxidase-independent extracellular H2O2 release. a H2O2 concentration in the media from BV-2 cells after stimulation with Cu(II)-Aβ1–40 (5 μM peptide), Aβ1–40, or Cu(II). b H2O2 concentration in the media from BV-2 cells treated with Cu(II)-Aβ1–40 (for 1 h) in the presence or absence of apocynin (100 μΜ) or DPI (10 μΜ). c Cu(II)-Aβ1–40 stimulation (for 1 h) had no obvious effect on extracellular superoxide release. Data are expressed as the means ± SEM of at least three independent experiments. Significance was tested by one-way or two-way ANOVA followed by Bonferroni post-test comparisons. *P < 0.05, **P < 0.01 vs control; ## P < 0.01 vs Aβ or Cu(II) alone