Fig. 7
From: Bee venom ameliorates lipopolysaccharide-induced memory loss by preventing NF-kappaB pathway
The expression of APP and BACE1 in astrocyte primary cells (a) and microglia BV-2 cells (b) were detected by western blotting using specific antibodies. Inhibitory effect of BV on LPS-induced NF-κB DNA-binding activity and on NF-κB-related protein in astrocyte primary cells (c) and microglia BV-2 cells (d). Activation of NF-κB was investigated using EMSA as described in the Methods section. Nuclear extracts were subjected to DNA-binding reaction with 32P end-labeled oligonucleotide specific to NF-κB. Specific DNA binding of the NF-κB complex is indicated by an arrow. The levels of Aβ1-42 in astrocyte primary cells (e) and microglia BV-2 cells (f) were measured by ELISA (n = 3). Values are presented as mean ± S.E. of the three independent experiments performed in triplicate. # p < 0.05 compared to control, *p < 0.05 compared to LPS. The expression of IκB, p-IκB, p50, and p65 in astrocyte primary cells (g) and microglia BV-2 cells (h) was detected by western blotting using specific antibodies. The expression of GFAP, IBA-1, COX-2, and iNOS in astrocyte primary cells (i) and microglia BV-2 cells (j) were detected by western blotting using specific antibodies. Each blot is representative of three experiments