Fig. 7
Expression of CD80, CD86 and MHCII in microglia, macrophages and dendritic cells is TNF dependent. TNFf/f (black circle), NsTNF−/− (triangle) and TNF−/− (clear circle) mice were infected with M. tuberculosis H37Rv at a dose of 1 × 103–1 × 104 CFUs/brain by intracerebral inoculation. Single-cell suspensions of brains at 0, 1, 2 and 3 weeks post-infection were generated and microglia (a–c), macrophages (d–f) and dendritic cells (g–i) analysed for expression of CD80 (a, d, f), CD86 (b, e, h) and MHC II (c, f, i). Data (mean ± SEM of the CFUs of 5–6 mice per time point) are representative of repeat experiments. **p ≤ 0.01