Fig. 2

Characterisation of the cytokine-secretion profiles of hCMVEC cultures following activation by IL-1β and TNFα. The concentration of each secreted cytokine/chemokine was measured in conditioned media using multiplex CBA. Shown are the temporal secretion profiles (4 to 72 h) of the 13 cytokines detected out of a larger screening panel of 38 factors (see Table 1 for full panel of cytokines). Most were negligible or undetectable under basal conditions with secretion increasing following treatment with either IL-1β (5 ng/mL) or TNFα (5 ng/mL) treatment. Data show the mean ± SEM (n = 3)