Fig. 4

Analysis of IL-1β and TNFα regulation of key adhesion molecules involved in leukocyte adhesion. a The flow plots show the cell-surface expression levels of adhesion molecules 24 h after cytokine treatment (5 ng/mL). Of this panel, IL-1β and TNFα treatments appear to substantially increase CD54 (ICAM-1) and CD106 (VCAM-1) expression only. Note also the bimodal distribution for CD106 following IL-1β activation. The black line denotes the autofluorescence of the cells. The blue curve represents the basal level of expression, whereas the red curve is the expression level following cytokine treatment. b Time course of selectin (CD62E, P and L) expression following cytokine activation of the hCMVECs. These data are from a single experiment, which is representative of at least three independent experiments