Pro-inflammatory TNFα and IL-1β differentially regulate the inflammatory phenotype of brain microvascular endothelial cells

Table 3 Expression of cell-surface adhesion molecules by brain endothelial cells

Molecules	Other names	Supporting literature	Expressed by the hCMVECs
CD102	ICAM2	Flow-cytometry analysis using hCMEC/D3 cells [34]	Yes
CD105	Endoglin	Flow cytometry using BMVEC [35]	Yes
CD106	VCAM1	RT-PCR using BMVEC [35]	Yes; highly inducible
CD138	Syndecan 1	Rat brain and rat brain-derived endothelial cells [36]	Yes
CD141	Thrombomodulin	IHC using human brain tissue (not expressed) [37]	Yes
CD141	Thrombomodulin	Regional distribution in human brain [38]	Yes
CD144	VE-cadherin	ICC using hCMEC/D3) [34], ICC using THBMEC [39]	Yes
CD144	VE-cadherin	IHC using human brain tissue [40]	Yes
CD146	MCAM; S-endo-1; MUC-18	Flow cytometry using BMVEC [35]	Yes
CD147	BSG		Yes
CD150	SLAMF1		No
CD304	Neuropilin-1	Functional assay using hCMEC/D3, and IHC-rat brain [41]	Yes
CD31	PECAM1	Flow cytometry using hCMEC/D3 [34]	Yes
		Flow cytometry and RT-PCR using BMVEC [35]
		Flow cytometry and ICC using HBMEC [42]
CD321	JAM1	ICC using hCMEC/D3[34], ICC using THBMEC [39]	Yes
CD321	JAM1	IHC on human brain microvessels [40]	Yes
CD325	Neural cadherin (NCAD)		Yes
CD34	HPCA-1	Flow cytometry using BMVEC [35]	Yes
CD44	H-CAM, Pgp-1	Flow cytometry using hCMEC/D3 [34]	Yes
CD49d	Alpha 4 integrin	THBMEC [39]	Yes
CD50	ICAM3	Expressed by leukocytes but not detected on endothelial cells in human brain by IHC [43]	Yes; low
CD54	ICAM1	Flow cytometry using hCMEC/D3 [34]	Yes; highly inducible
CD62E	E-selectin	ELISA detection using HCEC [44]	Yes; highly inducible
CD62L	L-selectin	Not expressed	No
CD62P	P-selectin	IHC using human brain [45];	Yes; very low
CD62P	P-selectin	IHC using human infarcted brain [46]	Yes; very low

hCMEC/D3 are immortalised human brain endothelial cell lines from adult females with epilepsy (not commercially available). BMVEC are human brain endothelial cells from foetal brain tissue. THBMEC are immortalised adult transfected human brain microvascular endothelial cells. HCEC are primary adult human cerebromicrovascular endothelial cells. HBMVE are primary human brain microvascular endothelia. HBMEC are human brain-derived microvascular endothelial cell (primary cells)
The better known generic names for each adhesion molecule are detailed alongside the literature demonstrating expression or function of the given adhesion molecule in endothelial cells or in brain tissue. The final column summarises whether the adhesion molecule was expressed by the hCMVECs used in this study
ICC immunocytochemistry, IHC immunohistochemistry

ISSN: 1742-2094