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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Transcript analysis of laser capture microdissected white matter astrocytes and higher phenol sulfotransferase 1A1 expression during autoimmune neuroinflammation

Fig. 2

Assessment of RNA integrity during immunohistochemical steps and laser microdissection procedures. a Microelectrophoresis of total RNA extracted from 8–12 coronal fresh-frozen spinal cord sections: (1) directly after quick defrosting (starting material), (2) after ethanol fixation, (3) at the end of the LCM immunohistochemistry steps in the presence of high NaCl concentration (high PBS), (4) with further 2 h dry at room temperature corresponding to the maximum time needed for drawing, dissecting, and capturing astrocytes on one slide, (5) after the microdissection of astrocytes, and (6) at the end of immunohistochemistry steps in the presence of isotonic NaCl concentration (normal PBS). b Example of an electrophoregram with RIN value corresponding to (5)

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