Fig. 3
From: STAT4 controls GM-CSF production by both Th1 and Th17 cells during EAE
STAT4 regulates GM-CSF production in a cell-intrinsic, lineage-independent manner. Naïve CD4 T cells from WT and STAT4−/− mice were activated under the indicated conditions for 6 days and then restimulated to assess GM-CSF production. a Representative GM-CSF staining gated on CD44hiCD4+ T cells for non-polarizing conditions are shown. b Publically available RNA-seq tracks comparing Csf2 transcripts from WT [Geo: GSM994535] and STAT4−/− [Geo: GSM994536] Th1 polarized cells, visualized using the IGV genome browser MM9 mouse gene database [17]. Arrows indicates gene direction. c–d Representative GM-CSF staining gated on CD44hiCD4+ T cells from c Th1 polarized and d Th17 polarized cells. e The ratio of GM-CSF+ CD44hiCD4+ T cells in the WT versus STAT4−/− cultures was quantitated. Data represent three independent experiments (mean ± SD). One-way ANOVA with Tukey’s multiple comparisons test was performed: **p < 0.01