Fig. 4
From: STAT4 controls GM-CSF production by both Th1 and Th17 cells during EAE
CD4 T cell-intrinsic expression of STAT4 is critical for production of GM-CSF during EAE. EAE was induced in WT:WT and WT:STAT4−/− mixed bone marrow chimeric mice. a Mice were scored daily for disease severity. b–c Spinal cord infiltrating lymphocytes were restimulated with MOG35–55 peptide and analyzed for IFNγ, IL-17A, and GM-CSF production. b Representative plots are gated on CD4+CD45.2+ T cells as well as GM-CSF+ (top) or GM-CSF− (bottom) cells. c The cumulative frequencies of IFNγ, IL-17A, and GM-CSF single, double, and triple cytokine-producing CD4+CD45.2+ T cells are shown. Data represent 2–7 independent experiments with (a) 3–5 or (b–c) 4–5 mice in each group (mean ± SD). Student’s t test was performed: ns = not significant; *p < 0.05; **p < 0.01