Fig. 5
From: STAT4 controls GM-CSF production by both Th1 and Th17 cells during EAE
STAT4 interacts with the Csf2 promoter and correlates with activation. a Publically available p300 ChIP-seq tracks from WT [Geo: GSM994508] and STAT4−/− [Geo: GSM994509] Th1 polarized cells were visualized for the Csf2 gene loci using IGV genome browser mouse MM9 database [17]. Arrows denote gene direction. b Conservation of the Csf2 promoter region between human and mouse. Predicted STAT4 binding sites are indicated by the green lines. Schematic and data were generated for this region by the rVista2.0 program (http://pipeline.lbl.gov/). c–d CD4 T cells from the spleens and dLNs of WT and STAT4−/− mice that had been previously immunized for EAE 10 days earlier were stimulated with MOG35–55 peptide and analyzed by ChIP. c STAT4 and (d) Pol II interactions with the Ifng and Csf2 promoter regions were assessed. Data are normalized to input control and quantitated relative to media stimulation. Data represent three independent experiments with 4–5 (pooled) mice in each group (mean ± SD). Student’s t test was performed: ns = not significant; *p < 0.05