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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Immune and Epstein-Barr virus gene expression in cerebrospinal fluid and peripheral blood mononuclear cells from patients with relapsing-remitting multiple sclerosis

Fig. 1

Improved sensitivity and specificity of PreAmp real-time RT-PCR for EBV transcript detection. a The expression levels of three EBV latent genes (EBNA1, LMP1, LMP2A) and one EBV lytic gene (BZLF1) were investigated in serially diluted EBV transformed (EBV+) LCL and in the EBV negative (EBV−) B lymphoma cell line BJAB, with and without pre-amplification (PreAmp), using Taqman self-designed gene assays. PreAmp resulted in an improvement of 4.1 to 5.1 cycles within the threshold Ct for low gene expression levels (≤35). EBV latent and lytic transcripts were detectable down to 1 and 10 EBV+ LCL cells, respectively; no signal was detected in EBV− BJAB cells confirming assay specificity. b Pre-Amp RT-PCR was applied to cDNA from EBV+ LCL cells that were serially diluted in a background of EBV− BJAB cells; the lower limits of detection of latent and lytic transcripts were one and two EBV+ LCL cells in 1 × 104 EBV− negative cells, respectively

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Journal of Neuroinflammation

ISSN: 1742-2094