Immune and Epstein-Barr virus gene expression in cerebrospinal fluid and peripheral blood mononuclear cells from patients with relapsing-remitting multiple sclerosis

Veroni, Caterina; Marnetto, Fabiana; Granieri, Letizia; Bertolotto, Antonio; Ballerini, Clara; Repice, Anna Maria; Schirru, Lucia; Coghe, Giancarlo; Cocco, Eleonora; Anastasiadou, Eleni; Puopolo, Maria; Aloisi, Francesca

doi:10.1186/s12974-015-0353-1

Journal of Neuroinflammation

Table 2 Expression of immune-related genes in CSF cells and PBMC from RRMS patients

From: Immune and Epstein-Barr virus gene expression in cerebrospinal fluid and peripheral blood mononuclear cells from patients with relapsing-remitting multiple sclerosis

Gene	CSF cell samples with detectable gene expression (%)	Relative amount in CSF cells, median (range)^a	PBMC samples with detectable gene expression (%)	Relative amount in PBMC, median (range)^a	Differences in gene expression between CSF cells and PBMC^b
CD20	100	0.28 (0.05–2.23)	100	0.39 (0.05–9.87)	n.s.
CD19	97	0.052 (0–0.18)	100	0.041 (0.007–0.18)	n.s.
CD138	94	0.033 (0–0.75)	79	0.00014 (0–0.0016)	<0.0001
BCMA	97	0.017 (0–0.15)	97	0.0019 (0–0.019)	<0.0001
CD4	100	0.74 (0.30–2.44)	100	0.45 (0.014–3.04)	0.0005
CD8	100	1.22 (0.31–4.93)	100	0.86 (0.04–2.27)	0.012
CD56	94	0.015 (0–0.026)	97	0.012 (0–0.90)	n.s.
NKp46	90	0.018 (0–0.083)	100	0.025 (0.002–0.129)	n.s.
CD68	100	0.29 (0.007–2.02)	100	0.77 (0.04–6.8)	0.0002
FoxP3	97	0.21 (0–2.42)	100	0.059 (0.00002–0.27)	0.0003
BDCA-2	97	0.057 (0–0.42)	100	0.019 (0.001–0.24)	0.0002
Perforin	100	0.18 (0.03–0.70)	100	0.21 (0.029–7.35)	n.s.
Granzyme B	97	0.014 (0–0.058)	100	0.068 (0.003–2.51)	<0.0001
MMP-9	84	0.006 (0–0.27)	100	0.19 (0.014–9.87)	<0.0001
IFN-γ	94	0.01 (0–0.079)	97	0.004 (0–0.089)	0.0064
TNF	100	0.10 (0.03–1.56)	100	0.064 (0.006–2.1)	0.02
IL-1β	97	0.089 (0–6.4)	100	0.45 (0.019–27.8)	0.02
IL- 2	84	0.0016 (0–0.015)	90	0.0007 (0–0.005)	0.03
IL-4	48	0 (0–0.0004)	69	0.00004 (0–0.0009)	0.004
IL-5	0	0	0	0	–
IL-6	84	0.0017 (0–0.14)	97	0.008 (0–0.16)	0.0015
IL-10	97	0.016 (0–0.22)	100	0.005 (0.0001–0.049)	0.0076
IL-15	87	0.026 (0–0.093)	97	0.02 (0–0.12)	n.s.
IL-17A	23	0 (0–0.0003)	41	0 (0–0.004)	n.s.
p40	77	0.0006 (0–0.046)	59	0.00001 (0–0.0015)	0.003
CXCL10	81	0.0008 (0–0.033)	93	0.0017 (0–0.43)	n.s.
CXCL13	81	0.002 (0–0.043)	66	0.00002 (0–0.0005)	<0.0001
IRF7	100	0.10 (0.027–0.64)	100	0.022 (0.001–1.65)	0.0006
ISG20	100	1.46 (0.17–6.32)	100	0.59 (0.06–7.0)	0.018
IFI6	100	0.18 (0.023–1.96)	100	0.10 (0.01–10.0)	n.s.
MxA	100	0.67 (0.11–6.20)	100	0.24 (0.046–14.39)	0.046
PKR	100	0.36 (0.058–1.91)	100	0.12 (0.006–1.39)	0.023
OAS1	100	0.05 (0.027–0.44)	100	0.038 (0.018–0.90)	n.s.
IFIT1	81	0.0006 (0–0.019)	90	0.001 (0–0.18)	0.0089
Usp18	94	0.008 (0–0.052)	93	0.003 (0–0.26)	0.0025
IFN-αR1	97	0.017 (0–0.053)	97	0.010 (0–0.18)	n.s.
BAFF	100	0.041 (0.010–0.21)	100	0.051 (0.014–1.03)	0.01
NAMPT	100	0.086 (0.016–0.72)	100	0.37 (0.02–19.9)	0.001
MHC class II	100	3.40 (0.66–9.13)	100	3.0 (0.18–92.1)	n.s.
iNOS	52	0.000007 (0–0.0015)	59	0.000005 (0–0.0004)	n.s.
COX-2	94	0.046 (0–0.26)	100	0.22 (0.007–2.15)	0.0008

n.s. not significant
^aGene expression values are presented as 2^-ΔCt relative to GAPDH. Data obtained in 31 CSF cell and 29 PBMC samples from 31 RRMS patients are shown
^bComparisons between paired CSF cell and PBMC samples (available for 29 patients) were made by Wilcoxon signed-rank test

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