Immune and Epstein-Barr virus gene expression in cerebrospinal fluid and peripheral blood mononuclear cells from patients with relapsing-remitting multiple sclerosis

Table 7 EBV gene expression levels and infection programs detected in CSF cells and PBMC from five RRMS patients

Patient code/sex, age	Time since diagnosis (months)	EDSS	Clinical condition	Gadolinium enhancement on MRI	IgG index	Cells/μl CSF	CSF					PBMC
Patient code/sex, age	Time since diagnosis (months)	EDSS	Clinical condition	Gadolinium enhancement on MRI	IgG index	Cells/μl CSF	EBV RNA speciesa	EBV infection program	CD19 RNA^a	CD20 RNA^a	GAPDH RNA (Ct)	EBV RNA species^a	EBV infection program	CD19 RNA^a	CD20 RNA^a	GAPDH RNA (Ct)
FI01/F 25 years	<1	1	Relapse	Present	0.90	2	n.d.	–	0.02	0.02	20.5	EBER1 0.00003	Latency 0 program	0.05	0.19	13.7
TO41/F 37 years	15	1	Remission	Absent	2.07	14	EBER1 0.00015	Latency II or default program	0.08	0.18	17.4	EBER1 0.000017	Latency 0 program	0.008	0.35	13.6
TO41/F 37 years	15	1	Remission	Absent	2.07	14	LMP2A 0.0001	Latency II or default program	0.08	0.18	17.4	EBER1 0.000017	Latency 0 program	0.008	0.35	13.6
TO32/F 35 years	5	2	Relapse	Present	0.67	10	LMP1 0.00023	Latency II or default program	0.05	0.13	18.2	n.d.	–	0.08	0.20	15.6
CA07/F 22 years	17	1	Remission	Absent	0.78	5	n.d.	–	0.13	0.19	19.7	LMP1 0.00008	Latency II or default program	0.04	0.37	16.8
FI13/M 51 years	<1	1	Relapse	Present	0.81	7	EBER1 0.0003	Lytic program	0.01	0.07	17.3	EBER1 0.00035	Latency III or growth program	0.07	11.7	18.6
							BZLF1 0.00009	(immediate early gene)				EBNA1 0.0004	Latency III or growth program
												EBNA3A 0.0004
												gp350/220 0.0012

Demographic, clinical, MRI, and CSF data of five RRMS patients with detectable EBV transcripts in CSF cells and/or PBMC are shown together with expression values of individual viral genes, B-cell related genes (CD19, CD20), and the housekeeping gene GAPDH in the corresponding samples
n.d. not detected
^aGene expression values are presented as 2^-ΔCt relative to GAPDH

ISSN: 1742-2094