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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: β-Lapachone suppresses neuroinflammation by modulating the expression of cytokines and matrix metalloproteinases in activated microglia

Fig. 3

β-LAP reduced neuroinflammation induced by systemic LPS administration. a Immunofluorescence labeling of Iba1 (red) and quantification of the number of activated Iba1-positive cells 3 h after systemic LPS treatment (5 mg/kg, i.p.). Nuclei are counterstained with DAPI (blue). Microglial activation in the cortex and dentate gyrus (DG) of LPS-injected mouse was reduced by β-LAP (10 mg/kg, i.p., daily for 4 days) treatment. Representative images were obtained from one set of experiments, and the three experiments were performed independently. Upper images are the results of Iba1 + DAPI staining with an original magnification of ×40. Lower images are the results of Iba1 staining with an original magnification of ×400. b, c β-LAP reduced the mRNA expression of proinflammatory cytokines (IL-6, IL-1β, TNF-α), iNOS, and MMPs (MMP-3, MMP-8, and MMP-9) in the cortex of LPS-injected mice (5 mg/kg, 3 h). Representative gels are shown in the left panel, and quantification data are shown in the right panel (n = 3 in each group). Results are representative RT-PCR data in the cortex 3 h after LPS treatment. Values are expressed as the means ± SEM for three independent experiments. *P < 0.05 vs. saline or # P < 0.005 vs. LPS-treated mice

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