Skip to main content
Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: β-Lapachone suppresses neuroinflammation by modulating the expression of cytokines and matrix metalloproteinases in activated microglia

Fig. 5

β-LAP inhibited the phosphorylation of MAPKs and AKT and DNA binding of NF-κB and AP-1 in LPS-stimulated BV2 cells. a Western blots for MAPKs and AKT activities. Cell extracts were prepared from BV2 cells pretreated with β-LAP (0.5, 1, and 2 μM, for 1 h), followed by LPS (100 ng/ml, for 1 h), and then subjected to immunoblot analysis using antibodies against the phospho- or total forms of JNK, ERK, p38 MAPK, and Akt. The autoradiograms are representative of three independent experiments. b Quantification of Western blot data. Levels of the phosphorylated forms of MAPKs and AKT were normalized with respect to the level of each total form and expressed as relative fold changes vs. the control group. Data are the means ± SEM for three independent experiments. *P < 0.05, significantly different from the LPS-treated samples. c, d EMSA for NF-κB and AP-1 DNA binding activity. BV2 cells were pretreated with β-LAP (0.5, 1, and 2 μM, for 1 h), followed by LPS (100 ng/ml, for 3 h), and nuclear extracts prepared from BV2 cells were incubated with the NF-κB (c) or AP-1 (d) probes. The arrow indicates a DNA–protein complex of NF-κB or AP-1. F indicates a free probe

Back to article page