Fig. 3

Diet-induced obesity increases chemokine expression and neutrophil number in the brain after stroke. Male C57BL/6 mice (8-week-old) were maintained on either a control or high-fat diet for 3 or 6 months. a In 6-month-fed mice, 30-min MCAo or sham surgery was performed. CXCL-1 and CCL3 protein expression were assessed in the striatum and cortex 24 h after reperfusion by CBA or ELISA, respectively. Dashed lines represent limit of detection. b In separate groups of mice, 30-min MCAo was performed after 3 and 6 months on diet. In mice fed diet for 6 months, MCAo was also performed for 20 min. The average number of neutrophils/section was assessed by immunohistochemistry after 24-h reperfusion. In (b) for this group of animals, the data for volume of ischaemic damage at 24 h is illustrated in Fig. 1b, d, e. Data are shown as mean ± standard error of the mean (SEM). For (a), *P < 0.05, **P < 0.01 versus sham group on the same diet, or versus control-fed mice for the same treatment; n = 5–6/group. Two-way ANOVA with Bonferroni’s. For (b), *P < 0.05 versus control-fed mice for the same brain region; n = 4–7/group. Student’s t-test