Fig. 4

The effect of a high-fat diet on microglia/macrophages in the brain after stroke. Male C57BL/6 mice (8-week-old) were maintained on either a control or high-fat diet and, in separate groups of mice, 30-min MCAo was performed after 3 (ai) and 6 (ci) months on diet. In mice fed diet for 6 months, MCAo was also performed for 20 min (bi). After 24 h, microglia and macrophages were stained immunohistochemically using Iba1. The area of Iba1-positive cells was analysed and expressed as a percentage increase from the contralateral hemisphere. For this group of animals, the data for volume of ischaemic damage at 24 h is illustrated in Fig. 1b, d, e. aii-cii Representative coronal brain sections are shown for microglia/macrophage cells on coronal brain sections. Magnification of the area highlighted by the dashed rectangle is illustrated in the insert. Scale bar, 1.0 mm and 200 μm for the inserts. Data are shown as mean ± standard error of the mean (SEM). n = 4–7/group. **P < 0.01 versus control-fed mice for the same brain region. Student’s t-test