Fig. 1

In OIR, GKT137831 reduced Iba1 immunolabeling of microglia and leukostasis. GKT137831 (GKT). a–c Representative 3-μm paraffin sections of retina immunolabeled with ionized calcium-binding adapter molecule 1 (Iba1, arrowheads). Iba1 immunolabeling is increased in OIR controls compared to room air controls, and in OIR controls, it is present in pre-retinal blood vessels (asterisk) protruding into the vitreous cavity. In OIR, treatment with GKT reduced Iba1 immunolabeling. Scale bar = 40 μm. GCL ganglion cell layer, IPL inner plexiform layer, INL inner nuclear layer. d Iba1 immunolabeling was quantitated in the inner retina (from retinal surface to IPL). n = 5 to 6 rats per group. e–g Representative whole mounts of retina from rats perfused with Concanavalin A to show leukocytes (arrowheads) adherent to the retinal vasculature. Leukostasis is increased in OIR controls compared to room air controls. In OIR, treatment with GKT reduced leukostasis. Scale bar = 40 μm. h Leukostasis was quantitated in the entire retina. n = 6 to 9 rats per group. **P < 0.01 to room air control. ##P < 0.01 to OIR control. Values are mean ± SEM