Fig. 3

In OIR, GKT137831 reduced Müller cell gliosis and vascular leakage in retina. GKT137831 (GKT). a–c Representative 3-μm paraffin sections of retina immunolabeled with glial fibrillary acidic protein (GFAP). GCL ganglion cell layer, INL inner nuclear layer, ONL outer nuclear layer. In room air controls, GFAP immunolabeling is detected at the retinal surface (asterisk), whilst in OIR controls, GFAP immunolabeling extends throughout the retina in Müller cell processes (arrowheads). In OIR rats treated with GKT137831, GFAP immunolabeling is reduced compared to OIR controls. Scale bar = 40 μm. d GFAP immunolabeling was quantitated in the entire retina. n = 5 to 6 rats per group. e Vascular leakage was measured in single retina by an albumin ELISA. n = 6 to 7 rats per group. *P < 0.05, **P < 0.01, and ***P < 0.001 to room air control. ##P < 0.01 to OIR control. Values are mean ± SEM