Fig. 1

Expression of CD300f and its ligand in normal and lesioned nerve. QPCR from lesioned nerve show time-dependent increased CD300f expression (a, relative to unlesioned nerve = 1). b-c, representative flow cytometry profiles showing the selection of macrophages in the crushed sciatic nerve at 3 dpl (b, CD45⁺CD11b⁺; c, CD45⁺CD11b⁺F480⁺), and d, expression of CD300f in CD45⁺CD11b⁺F480⁺ cells in the crushed sciatic nerve at 3 dpl (black, only secondary antibody-FITC; grey, hamster isotype control; green, hamster anti-CLM-1). e Quantification showing the percentage of macrophages expressing CD300f in the uninjured nerve and at 3, 10, and 28 dpl. Note that CD300f peaked at 3 dpl declining thereafter. Confocal images of teased fibres from adult sciatic nerves showed staining for the CD300f ligand (using CD300f-IgG2a), which did not co-localize with the axon (f, Thy1-YFP-H mice) nor with the MBP-positive myelin domain of myelinating Schwann cells (g). Partial co-localization could be observed with the S100-positive non-myelinating outer limit of the myelinating Schwann cells (h). The staining with CD300f-IgG2a in uninjured nerve (i) did not change at 10 dpl (j). IgG2a negative control did no show any staining (k). Scale bars: b: 30 μm; c, d: 100 μm; e-g: 20 μm