Fig. 3

Microglial repopulation following microglial elimination with PLX5622 and subsequent PLX5622 withdrawal. Two-month-old wild-type mice were treated with 1200 mg/kg chow PLX5622 or vehicle for 7 days. The PLX5622 was then removed and the number of microglia was assessed 3 days later. a–c IBA1/IB4 staining in control, 7 days treated with PLX5622, and 7 days treated with PLX5622 with 3 days recovery, with hippocampal region shown. d–f Whole brain sections were scanned and each IBA1⁺ cell counted. Representative brain sections are shown, with a white dot superimposed over each IBA1⁺ cell as a visual aid. g Quantification of d–f. *Indicates significance (p > 0.05) via one-way ANOVA with post hoc Newman-Keuls multiple comparison test. Error bars indicate SEM