Fig. 6

Activation of the inflammasome upon peripheral nerve injury. a Western blot analysis shows the induction of Nlrp3 and pro-IL-1β protein in axotomized sciatic nerves when compared to non-operated control nerves. b IL-1β protein expression in the injured sciatic nerve as determined by ELISA. c Western blot analysis of IL-1β secreted by Schwann cells stimulated with LPS and ATP. d Western blot analysis for caspase-1 protein in wild type and caspase-1/-11 knock-out mice. Intestine lysates of WT and caspase-1/-11-deficient mice that were treated with dextran sodium sulfate (DSS) (a known trigger for caspase-1 cleavage) were used as a positive control for the Western blot. e Western blot analysis shows increased activation of ERK1/2 upon peripheral nerve injury in Nlrp6^−/− mice compared to WT controls. For Western blot analysis, MW values expressed in kDa are shown at the right side of the blots. β-actin was used as a loading control