Fig. 2

NR1 phosphorylation was increased in the dorsal horn of the spinal cord during colitis. Up-regulation of p-NR1 in the spinal cord during colitis was examined by immunohistochemistry (a–d). The p-NR1 immunoreactivity was increased in the region of the spinal dorsal horn in L1 (a–b) and S1 (c–d) spinal cord during colitis (compare b to a and d to c, circled area). Histograms (e, f) summarized the changes in the relative density of p-NR1 immunoreactivity in the L1 (e) and S1 (f) spinal dorsal horn at 3 days and 7 days of colitis. Microphotographs were from 3 days of colitis and control animals. *p < 0.05 vs. control. Bar = 250 μm