Fig. 7
Inhibition of the PI3K in vivo with LY294002 blocked colitis-induced Akt activity and NR1 phosphorylation in the spinal cord. Animals receiving TNBS treatment (7 days) were divided into two groups: one receiving vehicle as control and one receiving LY294002 treatment. Animals treated with LY294002 had a reduced level of p-Akt in the spinal cord examined by western blot (a, b) and immunohistochemistry (c, d, circled areas are spinal dorsal horn), suggesting that inhibition of PI3K blocked Akt activity in vivo. Animals treated with LY294002 also had a reduced level of phospho-NR1 at Ser896 (e, f). Figure showed samples from S1 spinal cord. *p < 0.05. Bar = 250 μm