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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Osthole confers neuroprotection against cortical stab wound injury and attenuates secondary brain injury

Fig. 5

Osthole treatment promotes neuronal survival and inhibits apoptosis in the lesioned brain. a, d Neuronal survival in the lesioned cortex of mice was detected at 7 dpi by immunocytochemistry with antibodies against NF-M and NeuN. NF-M expression around the lesion was quantified by measuring pixel intensity of NF-M immunoreactivity using ImageJ software. Areas in boxes are shown at higher magnification in the insets. Scale bar, 250 μm in a, 25 μm in the insets. b Neuronal apoptosis as determined by the TUNEL assay. c Apoptotic neurons were labeled with anti-NeuN (green) and anti-caspase-3 (red) antibodies at 7 dpi; double positive cells indicated by arrows are shown at higher magnification in the insets. Scale bar, 25 μm in b and c, 10 μm in the insets. e Quantitative analysis of TUNEL-positive and NeuN/caspase-3 double positive cells. Nuclei were stained with DAPI (blue). Areas surrounding the lesion (Fig. 1b) were examined. Data represent mean ± SD (n = 6 per group). ## P < 0.01 vs. naive control; *P < 0.05, **P < 0.01, vs. SWI control

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