Fig. 6
From: Role of high-mobility group box 1 in methamphetamine-induced activation and migration of astrocytes
Methamphetamine induces HMGB1-mediated migration of astrocytes. The wound-healing assay showed that methamphetamine increased cell migration in a time-dependent manner in C6 cells (a) as well as in primary human astrocytes (b). c–d Transfection of C6 cells with siHMGB1 resulted in the inhibition of the methamphetamine-mediated induction of C6 cell migration. Scale bars all indicate 500 μm. The gap width from three separate experiments was quantitatively evaluated using Image J software. All the data are mean ± SD of three individual experiments. **p < 0.01 compared with control group; #p < 0.05 compared with methamphetamine-treated group in C6 cells