Fig. 1

Analysis of axonal mitochondrial morphology by transmission electron microscopy in cerebellar slice cultures. a Images of an organotypic cerebellar slice culture embedded in an Epoxy resin block (left image) and the white matter area selected for ultramicrotomy (area inside the black line, right image). b Segmentation method to study axonal mitochondrial morphology. Left image shows a section of cerebellar white matter with a longitudinally cut axon and a mitochondrion inside. The external mitochondrial perimeter (red line, right image) and the internal cristae perimeter (yellow line, right image) were drawn manually using ImageJ. Scale bars, 1 μm left image and 500 nm right image