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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: The disruption of mitochondrial axonal transport is an early event in neuroinflammation

Fig. 5

Effects of neuroinflammation and oxidative stress on mitochondrial function in cerebellar slice cultures. a Respiratory values of the cerebellar slice cultures show that neuroinflammation (LPS) produces an increase of LEAK, CI, CI + CII, uncoupling, and ETS I + II respiratory states, while oxidative stress (H2O2) causes an increase of LEAK and CI respiratory states (n = 13 controls, 9 LPS and 5 H2O2). One-way ANOVA and Dunnett’s multiple comparisons post hoc test for the respiratory states in which samples have a Gaussian distribution, Kruskal-Wallis test and Mann Whitney U test plus Bonferroni’s correction post hoc multiple comparisons for the respiratory states in which samples do not have a Gaussian distribution (mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001). b Respiratory values for mitochondrial complex IV were obtained in different experiments for LPS and H2O2 stimulations. Therefore, CIV values were normalized with the respiratory values of the negative control in each experiment. The resulting relative values show that only LPS produces an increase of CIV respiratory state (controls n = 12, LPS n = 9; H2O2 n = 7). One-way ANOVA and Dunnett’s multiple comparisons post hoc test (mean ± SEM; **p < 0.01). c Western blot showing the expression of two subunits from two different mitochondrial electron transport chain complexes in the organotypic cerebellar slice cultures: the 70-kDa Fp subunit of the succinate ubiquinone oxidoreductase or complex II (SUO); and subunit IV of the cytochrome c oxidase or complex IV (COX IV). d, e Quantification of the Western blot shows no significant differences in SUO or COX IV expression (n = 3, one-way ANOVA; mean ± SEM). f The LPS challenge significantly augments ATP production in the organotypic cerebellar slice cultures at 24 h (n = 3, two-way ANOVA and Dunnett’s multiple comparisons post hoc test; mean ± SEM; ***p < 0.001)

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