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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: The alternatively spliced fibronectin CS1 isoform regulates IL-17A levels and mechanical allodynia after peripheral nerve injury

Fig. 1

FN in CCI nerve. a Fibronectin (FN) exists as a heterodimer linked by disulfide bonds. One of the monomers includes the 25-amino-acid-long CS1 sequence. Typical CS1 inclusion and exclusion splice variants are illustrated. b FN immunoblot of rat sciatic nerve, normal or at days 1 and 7 after CCI. β-actin, a loading control (40 μg total protein). c The graph represents the mean levels of the individual FN isoform recorded in (b). ±SEM relative to β-actin (n = 3–4/group; *p < 0.05, **p < 0.01, n.s. not significant). d FN-CS1 immunostaining (red) in sciatic nerves at day 7 post-CCI. The vessel (V) endothelial cells, Schwann cells, and macrophages (confirmed using the CD68 immunostaining, green) are stained. DAPI, blue. Representative of n = 3/group; scale bars, 40 μm. e FN-CS1 immunostaining (red) in a teased nerve fiber at day 7 post-CCI; representative of ~20 individual fibers from n = 3. Scale bar, 10 μm. NoR node of Ranvier

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