Fig. 4From: Immune profile of an atypical EAE model in marmoset monkeys immunized with recombinant human myelin oligodendrocyte glycoprotein in incomplete Freund’s adjuvantPlasma IgG binding to native MOG. Necropsy plasma samples were tested for IgG binding with healthy marmoset myelin particles. a Gating strategy: myelin particles were plotted at a logarithmic FSC and SCC scale. Middle-sized particles were selected to determine the mean fluorescence intensity (MFI) in the FITC channel. b Marmoset myelin particles were incubated with 2000× diluted necropsy plasma samples, and IgG binding was detected. IgG binding to myelin was expressed as mean fluorescence activity (MFI). c Plasma samples of monkey M09083 were pre-incubated in two dilutions (1:500 and 1:5000) for 1 h at 37 °C with a dose titration of rhMOG to block anti-MOG IgG molecules. The pre-incubated plasma samples were subsequently tested for residual IgG reactivity with marmoset myelin particles. All data were corrected with background staining with the secondary onlyBack to article page