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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Immune profile of an atypical EAE model in marmoset monkeys immunized with recombinant human myelin oligodendrocyte glycoprotein in incomplete Freund’s adjuvant

Fig. 4

Plasma IgG binding to native MOG. Necropsy plasma samples were tested for IgG binding with healthy marmoset myelin particles. a Gating strategy: myelin particles were plotted at a logarithmic FSC and SCC scale. Middle-sized particles were selected to determine the mean fluorescence intensity (MFI) in the FITC channel. b Marmoset myelin particles were incubated with 2000× diluted necropsy plasma samples, and IgG binding was detected. IgG binding to myelin was expressed as mean fluorescence activity (MFI). c Plasma samples of monkey M09083 were pre-incubated in two dilutions (1:500 and 1:5000) for 1 h at 37 °C with a dose titration of rhMOG to block anti-MOG IgG molecules. The pre-incubated plasma samples were subsequently tested for residual IgG reactivity with marmoset myelin particles. All data were corrected with background staining with the secondary only

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