Fig. 1

Expression of 14-3-3 γ in glial cells. a RT-PCR analyses of spinal cord for the presence of 14-3-3 γ mRNA (n = 4 mice per time point). The relative expression in the spinal cord of naïve mice is set to 1. Note the increase of 14-3-3 γ expression in the spinal cord during EAE, particularly at later time points (*p < 0.05 at day 78 of EAE). b RT-PCR analysis of 14-3-3 γ mRNA in glial cells as compared to naïve spinal cord (n = 4 for spinal cord versus n = 9 independent cell preparations for astrocytes and n = 3 independent preparations for OPC). Note the significant 14-3-3 γ mRNA expression in glial cells as compared to total spinal cord (**p < 0.01). c, d Representative confocal laser scanning images after immunolabeling for 14-3-3 γ with GFAP in primary astrocyte culture c or Olig2 in primary OPC culture d shows expression of 14-3-3 γ on the protein level in the cytoplasm of some, but not all cells (arrows depict double labelled cells, bar = 20 μm)