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Fig. 10 | Journal of Neuroinflammation

Fig. 10

From: Exposure of neonatal rats to alcohol has differential effects on neuroinflammation and neuronal survival in the cerebellum and hippocampus

Fig. 10

Postnatal alcohol exposure (PAE) increases astrocytic glial fibrillary acidic protein (GFAP) expression in the hippocampus. Representative images of the hippocampus are stained for GFAP (red) to label astrocytes and 4′,6-diamidino-2-phenylindole (DAPI, blue) to label cell nuclei during the first withdrawal period on P4 (ad), the third withdrawal period on P6 (eh), and also on P45 (il) (as shown in Fig. 1a). The CA1 and CA3 subregions the stratum oriens (SO), stratum pyramidale (SP) and either the stratum radiatum (SR), and stratum lacunosum moleculare (SLM) layers or the stratum lucidum (SL), respectively, were traced and GFAP intensity was quantified within. In the dentate gyrus (DG), the molecular layer (ML), granule cell layer (GCL), and hilus were quantified. (*p < 0.05, **p < 0.01, ***p < 0.001). n = 4 animals from 4 litters. Scale bars are 40 and 10 μm for low and high magnification images, respectively. SP of the CA1 region and the GCL of the DG are labeled in the images

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