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Table 1 Effects of cell-type-specific ablation of NG2 on cell and axon biology

From: NG2-proteoglycan-dependent contributions of oligodendrocyte progenitors and myeloid cells to myelin damage and repair

Row   Control OPC-NG2ko My-NG2ko
  Sham-operated animals    
1  Number of Olig2+ cells 115.27 ± 3.8 111.02 ± 5.7 117.63 ± 5.4
2  Number of Olig2+/PDGF-Rα+ OPCs 14.55 ± 1.7 12.31 ± 2 14.46 ± 3.5
  1 week after demyelination    
3  Number of Iba1+ macrophages/microglia 117.19 ± 22.3 120.1 ± 28.1 39.64 ± 19.8**
4  Number of Olig2+/PDGF-Rα+ OPCs 42.92 ± 1.5 32.4 ± 1.7*** 23.02 ± 7.2***
5  Mitotic index of Olig2+/PDGF-Rα+ OPCs (%) 22.91 ± 3.5 13.49 ± 1.5** 11.93 ± 2.1***
  6 weeks after demyelination    
6  Number of APC+ oligodendrocytes 62.14 ± 6.7 44.48 ± 8.6* 43.17 ± 4.1**
7  Well-myelinated axons 1056 ± 133 480 ± 128** 499 ± 80**
8  Myelin thickness (g value) 0.745 ± 0.089 0.861 ± 0.099*** 0.87 ± 0.065***
9 Number of NF-positive axons    
   Small: diameter < 1 μm 1103 ± 60 660 ± 66*** 812 ± 85**
   Medium: diameter = 1–2.5 μm 201 ± 29 135 ± 10* 118 ± 14*
   Large: diameter > 2.5 μm 18 ± 3 6 ± 1** 8 ± 2*
  1. The key parameters for Olig2/PDGFRα-positive OPCs, Iba1-positive macrophages/microglia, and APC-positive oligodendrocytes were quantified in lesions in control, OPC-NG2ko, and My-NG2ko mice (n = 4 for each group) from 1 to 6 weeks after lysolecithin injection. The rationale for using Iba1 labeling in place of CD18 labeling was to attempt quantification of both activated myeloid cells inside lesions and non-activated myeloid cells remaining outside lesions. However, in this set of results, we have only examined Iba1-positive cells within lesions, in parallel to analyses of lesional CD18-positive cells in other parts of the text. Values represent means ± S.D. Statistically significant differences evaluated by ANOVA and t-test are indicated by * P < 0.05, ** P < 0.01, and *** P < 0.001 when values were compared between controls and NG2 conditional knockout mice at the same time point. Cell and axon numbers are determined in 100,000-μm3 volumes.