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Table 1 Effects of cell-type-specific ablation of NG2 on cell and axon biology

From: NG2-proteoglycan-dependent contributions of oligodendrocyte progenitors and myeloid cells to myelin damage and repair

Row

 

Control

OPC-NG2ko

My-NG2ko

 

Sham-operated animals

   

1

 Number of Olig2+ cells

115.27 ± 3.8

111.02 ± 5.7

117.63 ± 5.4

2

 Number of Olig2+/PDGF-Rα+ OPCs

14.55 ± 1.7

12.31 ± 2

14.46 ± 3.5

 

1 week after demyelination

   

3

 Number of Iba1+ macrophages/microglia

117.19 ± 22.3

120.1 ± 28.1

39.64 ± 19.8**

4

 Number of Olig2+/PDGF-Rα+ OPCs

42.92 ± 1.5

32.4 ± 1.7***

23.02 ± 7.2***

5

 Mitotic index of Olig2+/PDGF-Rα+ OPCs (%)

22.91 ± 3.5

13.49 ± 1.5**

11.93 ± 2.1***

 

6 weeks after demyelination

   

6

 Number of APC+ oligodendrocytes

62.14 ± 6.7

44.48 ± 8.6*

43.17 ± 4.1**

7

 Well-myelinated axons

1056 ± 133

480 ± 128**

499 ± 80**

8

 Myelin thickness (g value)

0.745 ± 0.089

0.861 ± 0.099***

0.87 ± 0.065***

9

Number of NF-positive axons

   
 

 Small: diameter < 1 μm

1103 ± 60

660 ± 66***

812 ± 85**

 

 Medium: diameter = 1–2.5 μm

201 ± 29

135 ± 10*

118 ± 14*

 

 Large: diameter > 2.5 μm

18 ± 3

6 ± 1**

8 ± 2*

  1. The key parameters for Olig2/PDGFRα-positive OPCs, Iba1-positive macrophages/microglia, and APC-positive oligodendrocytes were quantified in lesions in control, OPC-NG2ko, and My-NG2ko mice (n = 4 for each group) from 1 to 6 weeks after lysolecithin injection. The rationale for using Iba1 labeling in place of CD18 labeling was to attempt quantification of both activated myeloid cells inside lesions and non-activated myeloid cells remaining outside lesions. However, in this set of results, we have only examined Iba1-positive cells within lesions, in parallel to analyses of lesional CD18-positive cells in other parts of the text. Values represent means ± S.D. Statistically significant differences evaluated by ANOVA and t-test are indicated by * P < 0.05, ** P < 0.01, and *** P < 0.001 when values were compared between controls and NG2 conditional knockout mice at the same time point. Cell and axon numbers are determined in 100,000-μm3 volumes.