Fig. 4
Blockade of Fas signaling on retinal cells in Wt mice inhibits HMGB1 release and tEAU induction. a, b Retinal explants (or R) from Wt mice were cultured for 6 h with or without Jo2 (a) or activated IRBP1–20-specific T cells (b) in the presence or absence of 1 μg/ml of Met 12 (a, b) or the mutant Met 12 (m) (b), then HMGB1 levels in the culture supernatant were measured by ELISA. **p < 0.01 compared to retinal explants treated with medium by one-way ANOVA. c, d Wt mice injected with IRBP1–20-specific T cells were injected systemically (S) or intravitreously (L) with Met 12 or PBS (Ctrl) and examined on day 15; c shows the pathological score for each group (n = 12 mice) presented as the mean ± SEM, *p < 0.05 compared to PBS treatment using the Mann-Whitney U test, while d shows representative ocular histopathology after H & E staining, original magnification, ×100