Fig. 3
Acute inhibition of NOX2 using gp91ds-tat reduces p47PHOX expression and/or phosphorylation. Phosphorylated p47PHOX (p-p47) and GAPDH were evaluated at 24 h, 7 days, and 28 days post-injury in naïve (N; n = 8), scrambled ds-tat- (S; n = 4/24 h; 6/7 days; 3/28 days), and gp91ds-tat-treated samples (G; n = 4/24 h; 5/7 days; 3/28 days). Bands were observed at 47 and 38 kDa, respectively, in a representative western blot (a). Pixel densitometry for p-p47PHOX (b) showed significant induction of phosphorylation of p47PHOX at 24 h. NOX2 inhibition reduced p-p47PHOX levels at 7 days to a point that was not significantly greater than sham. **p < 0.01 vs naïve. +p < 0.01 vs scrambled; one-way ANOVA with Tukey’s post-test. Bars represent mean ± SEM