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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Inhibition of NOX2 reduces locomotor impairment, inflammation, and oxidative stress after spinal cord injury

Fig. 7

Acute NOX2 inhibition reduces microglia/macrophage presence in injured spinal cord tissue. Gp91ds-tat- and scrambled ds-tat-treated tissue at 24 h (a, b; n = 4/group) and 7 days (c, d; n = 3 gp91ds-tat, 4 scrambled ds-tat) post-injury was stained for the pan microglia/macrophage marker Iba1 (red). DAPI nuclear stain is shown in blue. At 24 h post-injury, the scrambled ds-tat-treated tissue (a) have a greater number of activated microglia compared to gp91ds-tat-treated tissue as seen in ×10 magnification (b). The activated morphology includes a more amoeboid body with retracted dendrites (which can be better observed in the higher magnification inset image (×20)). At the same time point, the microglia present in gp91ds-tat tissue have long processes and smaller bodies. This difference is greater at the 7-day time point when microglia are at their peak (c, d). The gp91ds-tat-treated microglia at 7 days (×20) are activated, but the number are still downregulated compared to those treated with scrambled-tat. Lower magnification and high-magnification images shown; bar (×20) = 50 μm; bar (×10) = 100 μm

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