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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Interleukin-36γ is expressed by neutrophils and can activate microglia, but has no role in experimental autoimmune encephalomyelitis

Fig. 3

IL-36γ stimulates the expression of inflammatory genes in microglial cultures. a Genes that were upregulated ≥2.5 times in BV2 microglia treated for 6 h with recombinant IL-36γ (100 ng/ml) compared to PBS, as determined using Affymetrix DNA microarrays (Student’s t test, P < 0.04). b, c Quantitative PCR analysis confirming that Csf3, IL-1β, and Cxcl2 mRNAs were upregulated by IL-36γ in BV2 cells (b) and primary microglia from IL-36R+/+ mice (c) (Wilcoxon test, P < 0.005). Note in c the absence of upregulation in primary microglia from IL-36R−/− mice, confirming the specificity of the results

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