Fig. 5

No difference in leukocyte recruitment in the spinal cord of EAE mice expressing or not IL-36γ or IL-36R. a Gating strategy used for flow cytometric analysis. Dead cells and doublets were excluded. The data shown are from a representative wild-type EAE mouse. b, c Counts of immune cells in the spinal cord of IL-36γ^−/−, IL-36R^−/−, or wild-type mice killed 21 days after immunization with MOG peptide. The counts were normalized to CD45⁻ cells used as an internal control. When the cells were collected, the animals had clinical scores ranging from 0.5 to 3. No significant difference was found between the genotypes (Wilcoxon tests, P ≥ 0.07). Sample size: 6 (b) or 9 (c) mice per group