Fig. 7

Effects of IL-17A on microglial activation and cytokine production. Primary microglial cells were incubated with IL-17A at 1, 10, and 100 ng/ml for 24 h. a The cells were stained with an Iba1 antibody. Upregulated Iba1 expression (green) in activated microglia was observed using confocal scanning. The blue staining represents DAPI. Scale bar = 50 μm. b Graph showing the mean fluorescence intensity (MFI) for Iba1. c, d Quantification of TNF-α and IL-6 in the media. The data are presented as the mean ± s.e.m. of four independent experiments. **P < 0.01 versus the response to medium alone