Fig. 4
From: NT-020 treatment reduces inflammation and augments Nrf-2 and Wnt signaling in aged rats
Bar graph summarizing the percent nuclear localization of Nrf2, HO-1, and β-catenin. For each condition, at least 100 cells were counted except for the aged DCX condition where this was not possible due to low cell counts. As can be seen, NT-020 treatment increases the percent of cells with nuclear expression of all three proteins in all four cell types independent of the age of the rats. (One-way ANOVA followed by Tukey’s post hoc analysis ***p < 0.001 for treatment versus age-matched control condition. DCX/β-catenin F = 20.63, df = 3; DCX/Nrf2 F = 12.69, df = 3; DCX/HO-1 F = 26.87, df = 3; GFAP/β-catenin F = 86.53, df = 3; GFAP/Nrf2 F = 65.37, df = 3; GFAP/HO-1 F = 107.9, df = 3; NeuN/β-catenin F = 77.1, df = 3; NeuN/Nrf2 F = 190.7, df = 3; NeuN/HO-1 F = 165.3, df = 3; IBA-1/β-catenin F = 144.1, df = 3; IBA-1/Nrf2 F = 86.3, df = 3; IBA-1/HO-1 F = 84.16, df = 3). Baseline nuclear localization of β-catenin was observed in mature neurons and microglia in aged rats compared with young rats, p < 0.05 (Tukey’s multiple comparison test). Basal nuclear localization of HO-1 was only increased in the astrocytes in the aged rats, p < 0.05 (Tukey’s multiple comparison test)